![]() There was no difference between total collection and internal marker methods in the assessment of in vivo digestibility for any of the nutrients considered. On the contrary, fiber digestibility was lower in the insect-based diet compared with the venison diet ( p < 0.001). Calcium digestibility was higher in the BSF diet compared with the CTRL diet ( p = 0.018). However, a statistical trend ( p = 0.066) was observed indicating greater protein digestibility in the BSF diet compared with the CTRL diet. The two diets showed similar nutrient digestibility values for dry matter, organic matter, ether extract, ash, and phosphorus. The digestibility of nutrients was determined using both in vivo (“total collection” and “internal marker” approaches) and in vitro methods. Two isonitrogenous and isoenergetic dry diets containing either venison meal (CTRL diet) or black soldier fly larvae meal (BSF diet) as their primary sources of proteins were fed to six adult dogs, according to a Latin square design. Moreover, the purpose of the study was to compare the traditional in vivo total collection method with the in vivo marker method and in vitro digestibility method. The aim of the study was to evaluate nutrient digestibility of a diet containing black soldier fly larvae as its main protein source. Growing attention is being directed toward insects as a novel and sustainable source of protein for pet food. ![]() 3Department of Animal Production, University of Murcia, Murcia, Spain.2Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. ![]() 1Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.Livio Penazzi 1, Achille Schiavone 1 *, Natalia Russo 1,2, Joana Nery 1, Emanuela Valle 1, Josefa Madrid 3, Silvia Martinez 3, Fuensanta Hernandez 3, Elena Pagani 1, Ugo Ala 1 and Liviana Prola 1
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